A test for chapter 1

1、多选题：
​The research contents of Synthetic Biology include‌
选项：
A: Synthesis and modularization of biomacromolecules.
B: synthesis, simplification and reconstruction of biological genomes.
C: systhesis of anabolic network.
D: design and construction of genetic circuits.
E: cell population system and multicellular system.
答案: 【 Synthesis and modularization of biomacromolecules.;
synthesis, simplification and reconstruction of biological genomes.;
systhesis of anabolic network.;
design and construction of genetic circuits.;
cell population system and multicellular system.】

2、多选题：
‍The 3 main features of Synthetic Biology are‎
选项：
A: characteristics of engineering.
B: Extensive use of mathematical simulation tools.
C: Features of standard parts.
D: characteristics of contribution and sharing.
E: network analysis is one of its core contents.
答案: 【 characteristics of engineering.;
Features of standard parts.;
characteristics of contribution and sharing.】

3、多选题：
‎Compared with Genetic Engineering, the features of Synthetic Biology include ‌
选项：
A: Characteristics of engineering.
B: Extensive use of mathematical simulation tools.
C: Features of standardized parts.
D: Characteristics of interdisciplinary subject.
E: Network analysis is one of its core contents.
答案: 【 Extensive use of mathematical simulation tools.;
Features of standardized parts.;
Characteristics of interdisciplinary subject.;
Network analysis is one of its core contents.】

4、多选题：
‍What are the applications of Synthetic Biology in the field of biomedicine?‍
选项：
A: development natural medicines.
B: design immune cells.
C: development of diagnostic kits.
D: development and production of vaccines.
E: individualized medicine.
答案: 【 development natural medicines.;
design immune cells.;
development of diagnostic kits.;
development and production of vaccines.;
individualized medicine.】

5、多选题：
‌What does biosafety include?‍
选项：
A: The harm caused to researchers by the leakage of synthetic organisms.
B: Damage to the public environment caused by leakage of synthetic organisms.
C: The use of Synthetic Biotechnology to synthesize deadly pathogens for use in terrorist attacks.
D: Biochemical warfare with synthetic biotechnology.
E: Synthesis of new or extinct species by means of synthetic biotechnology to increase biodiversity.
答案: 【 The harm caused to researchers by the leakage of synthetic organisms.;
Damage to the public environment caused by leakage of synthetic organisms.;
The use of Synthetic Biotechnology to synthesize deadly pathogens for use in terrorist attacks.;
Biochemical warfare with synthetic biotechnology.】

6、多选题：
​Principles proposed by the President’s committee on bioethics for the development of Synthetic Biology include ‍
选项：
A: Principle of public benefit
B: principle of responsibility management
C: principles of academic freedom and responsibility
D: principle of democratic evaluation
E: principles of fairness and justice
答案: 【 Principle of public benefit;
principle of responsibility management;
principles of academic freedom and responsibility;
principle of democratic evaluation;
principles of fairness and justice】

7、判断题：
‎According to function, genetic circuits can be divided into logical genetic circuits and functional genetic circuits.‎
选项：
A: 正确
B: 错误
答案: 【 正确】

8、判断题：
‍Synthetic Biology can design and construct biological systems that do not exist in nature.‌
选项：
A: 正确
B: 错误
答案: 【 正确】

9、判断题：
‍Synthetic Biology is a typical paradigm of the convergence research.‌
选项：
A: 正确
B: 错误
答案: 【 正确】

10、判断题：
‎Convergence technology is a new method in the field of biomedicine.‌
选项：
A: 正确
B: 错误
答案: 【 正确】

11、判断题：
‏Synthetic Biology has huge potential dangers, such as making biochemical weapons, like viruses, so human should not develop it.​
选项：
A: 正确
B: 错误
答案: 【 错误】

12、判断题：
‎Once the knowledge of Synthetic Biology is abused by people with ulterior motives, if it is not the will of the scientist, the scientist can take no responsibility.​
选项：
A: 正确
B: 错误
答案: 【 错误】

A test for chapter 2

1、单选题：
‍What kind of genes are usually initiated for expression by constitutive promoter?​
选项：
A: reporter gene
B: luxury gene 
C: housekeeping gene
D: resistance gene 
E: stress protein gene
答案: 【 housekeeping gene】

2、单选题：
‌The regulatory sequence binding to a repressor protein is called‍
选项：
A: RBS 
B: operon 
C: promoter 
D: operator 
E: SD sequence
答案: 【 operator 】

3、单选题：
‍The wrong statement about the ribosomal binding site is:‎
选项：
A: The ribosomal binding site, abbreviated as RBS, is an RNA sequence that binds to ribosomes.
B: The ribosomal binding site in prokaryotes is also known as SD sequence.
C: The ribosomal binding site in prokaryotes is rich in purine structure and can bind to the 16srRNA 3 ‘-end rich in pyrimidines.
D: The strength of the binding of RBS to the ribosome depends on its own structure and the distance between it and AUG.
E: Ribosomal binding sites can initiate gene transcription.
答案: 【 Ribosomal binding sites can initiate gene transcription.】

4、单选题：
‌Regarding the description of system, which one is wrong? ‎
选项：
A: System is a more complex cascade of multiple devices, or regulatory network.
B: Different devices can be physically connected in series, parallel, feedforward and feedback to form a System.
C: Transcriptional level regulation network is a common system.
D: Gene oscillator, pulse generator and feedback loop belong to the systems.
E: Several biological modules can form a system.
答案: 【 Different devices can be physically connected in series, parallel, feedforward and feedback to form a System.】

5、单选题：
‍About the descriptions of biological module, which one is wrong? ‌
选项：
A: Biological module is a set of intracellular localized biological devices.
B: Biological module performs specific complex functions.
C: Like part, device, and system, the biological module is a hierarchy inside cells.
D: Biological module is a concept proposed to reduce the complexity of synthetic biological systems. It emphasizes "functional independence".
E: Biological modules in cells are often pathways with specific functions, such as metabolic pathway or signal transduction pathway.
答案: 【 Like part, device, and system, the biological module is a hierarchy inside cells.】

6、单选题：
‎Which level is RIPS used for a standardized measurement? ‍
选项：
A: transcriptional level 
B: translational level 
C: metabolic level  
D: replication level 
E: signal transmission level
答案: 【 translational level 】

7、多选题：
Standardization of biological systems includes ​
选项：
A: Physical standardization of assembly parts
B: Standardization of quantitative analysis of biological functions
C: Registry of standard parts
D: Standardization of decoupling methods
E: Standardization of system operation
答案: 【 Physical standardization of assembly parts;
Standardization of quantitative analysis of biological functions;
Registry of standard parts;
Standardization of system operation】

8、多选题：
The concepts involved in the engineering research strategy of Synthetic Biology are ​
选项：
A:  standardization 
B: hierarchy
C: decoupling  
D: connection  
E: abstraction
答案: 【  standardization ;
decoupling  ;
abstraction】

9、多选题：
‌Some tools of Synthetic Biology which are different from that of Genetic Engineering include:‏
选项：
A: isocaudamer
B:  PCR
C: de novo synthesis of DNA
D: standardization  
E: abstraction
答案: 【 de novo synthesis of DNA;
standardization  ;
abstraction】

10、多选题：
The hierarchy of synthetic biological systems include​
选项：
A: Module
B: Part 
C: System
D: BioBrick 
E: Device
答案: 【 Part ;
System;
Device】

11、多选题：
In the followings, which do you think are parts?‍
选项：
A: reporter gene 
B: replicon 
C: restriction site
D: selective marker
E: prim